FIGURE

Fig. 3

ID

ZDB-FIG-240112-37

Publication

Mahony et al., 2023 - Lineage skewing and genome instability underlie marrow failure in a zebrafish model of GATA2 deficiency

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Fig. 3

Differential regulation of chromatin accessibility and myeloid and erythroid gene expression by Gata2a in cHSPCs and mHSPCs (A and B) Volcano plots of log2 fold change in gene expression vs. –log10(p value) in mHSPCs and cHSPCs. Each dot represents a gene. Dotted line indicates p = 0.05 and fold change = 0.25 with black genes falling below this threshold. (C) GO term enrichment (performed using https://metascape.org ) analysis of up- or downregulated genes in mHSPCs with a p <0.05. mHSPCs, myeloid HSPCs. Full list of differentially expressed genes can be found in Table S2. (D and E) Chromatin accessibility near promoter regions (scATAC-seq) and RNA expression (scRNA-seq) of indicated genes in either cHSPCs (D) or mHSPCs (E). In (D), WT vs. gata2a−/−: cebpa p = 3.36E−10; npm1a p > 0.05; lcp1 p = 3.32E−15; hbba1.1 p = 3.72E−18; hemgn p > 0.05. In (E), WT vs. gata2a−/−: cebpa p = 0.00039; npm1a p = 0.034; lcp1 p = 1.5E−15; hbba1.1 p = 8.13E−8; hemgn p = 0.00085. cHSPC, core HSPC. mHSPCs, myeloid HSPCs. NS p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. See also Figure S3 and Table S2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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