Fig. 2

Pharmacologic inhibition of mitochondrial function phenocopies the mrpl40 mutant phenotype

A Chloramphenicol experimental setup. Animals were treated daily with chloramphenicol in E3 or with E3 alone and then behaviorally tested at 6dpf. B Heatmap illustrating behavioral phenotype across VMR, DF, and ASR behavioral metrics based on mrpl40 genotype and Chloramphenicol (Cam) treatment status. mut+E3 (n = 27), wt+Cam (n = 18), het+Cam (n = 41), mut+Cam (n = 19) from two biological replicates. Values that are significantly different from sibling/untreated controls based on Student’s t test with a p-value of <0.05 are designated with #. See Fig. 1 legend for full description of heatmap. C Chloramphenicol-treated larvae have dose-dependent reductions in the average duration of movement following DF stimuli. E3 alone (n = 30), 375 μM (n = 13), 562 μM (n = 29), 750 μM (n = 15) from three biological replicates. One-way ANOVA, p-values are displayed on the plot. D Percent Acoustic Startle Response based on mrpl40 genotype and Cam treatment status. mrpl40 heterozygotes and mutants are hypersensitive to Cam-treatment. Wildtypes (n = 12), hetererozygotes (n = 40), mutants (n = 27) with E3. Wildtypes (n = 18), hetererozygotes (n = 41), mutants (n = 19) with Chloramphenicol 562 μM from two biological replicates.. One-way ANOVA, p-values are displayed on the plot.