Fig. 5

Ovarian growth and follicle development in the triple mutant of cyp19a1a, nobox and dmrt1.

a Gross morphology of the ovaries (arrow) in females of the control (nobox+/−;cyp19a1a+/−;dmrt1+/−, n = 3 independent fish), cyp19a1a and dmrt1 double mutant (nobox+/−;cyp19a1a−/−;dmrt1−/−, n = 3 independent fish), and nobox and dmrt1 double mutant (nobox−/−;cyp19a1a+/−;dmrt1−/−, n = 3 independent fish). b Ovarian histology and secondary sex characteristics in different genotypes: control (nobox+/−;cyp19a1a+/−;dmrt1+/−, n = 3 independent fish), cyp19a1a and dmrt1 double mutant (nobox+/−;cyp19a1a−/−;dmrt1−/−, n = 3), nobox and dmrt1 double mutant (nobox−/−;cyp19a1a+/−; dmrt1−/−, n = 3 independent fish), and nobox, cyp19a1a and dmrt1 triple mutant (nobox−/−;cyp19a1a−/−; dmrt1−/−, n = 3 independent fish). Red asterisk, breeding tubercles; arrowhead, genital papilla; black asterisk, vacuoles left by the lost oocytes. c, d Body weight and body length of the triple mutant of cyp19a1a, nobox and dmrt1 (n = 6 independent fish). Data shown are mean ± SEM, P values revealed by one-way ANOVA and Tukey’s test.