FIGURE

Figure 1

ID

ZDB-FIG-231025-5

Publication

Lu et al., 2023 - Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples

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Figure 1

Comprehensive Workflow for Analyzing Peptide, Steroid, and Protein Components in Pituitary Samples. (A) Overall sample processing workflow from tissue homogenate to specific samples for corresponding analyses. (B) Chromatogram and mass spectrometry results for CGA and LHB) peptides selected for targeted proteome analysis. (C) Chromatogram and mass spectrometry results for selected peptide and steroid analyses, with AVP, GNRH3, and testosterone serving as model compounds among all analytes. (D) 93 pituitary proteins (5.1%) that were identified with both peptidomics and trypsin-digested proteomics. Also shown are the numbers of other peptides (75; 4.1%) and proteins (1666; 90.8%) identified.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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