ZFIN Image: Lu et al., 2023, Figure 1

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Figure 1

Comprehensive Workflow for Analyzing Peptide, Steroid, and Protein Components in Pituitary Samples. (A) Overall sample processing workflow from tissue homogenate to specific samples for corresponding analyses. (B) Chromatogram and mass spectrometry results for CGA and LHB) peptides selected for targeted proteome analysis. (C) Chromatogram and mass spectrometry results for selected peptide and steroid analyses, with AVP, GNRH3, and testosterone serving as model compounds among all analytes. (D) 93 pituitary proteins (5.1%) that were identified with both peptidomics and trypsin-digested proteomics. Also shown are the numbers of other peptides (75; 4.1%) and proteins (1666; 90.8%) identified.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)