PUBLICATION
Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples
- Authors
- Lu, C., Peng, D., Erandani, W.C.K.U., Mitchell, K., Martyniuk, C.J., Trudeau, V.L.
- ID
- ZDB-PUB-231025-60
- Date
- 2023
- Source
- Frontiers in endocrinology 14: 12669851266985 (Journal)
- Registered Authors
- Trudeau, V.L.
- Keywords
- LCMS (liquid chromatography-mass spectrometry), peptide quantification, protein measurement, solid phase extraction, steroid detection
- MeSH Terms
-
- Animals
- Hormones
- Humans
- Peptides
- Steroids/metabolism
- Tandem Mass Spectrometry*/methods
- Zebrafish*/metabolism
- PubMed
- 37876537 Full text @ Front Endocrinol (Lausanne)
Citation
Lu, C., Peng, D., Erandani, W.C.K.U., Mitchell, K., Martyniuk, C.J., Trudeau, V.L. (2023) Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples. Frontiers in endocrinology. 14:12669851266985.
Abstract
The detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using different extraction methodologies. With the new advancement on HPLC sorbents, the simultaneous measurement of hormones from different categories becomes possible. In this study, we present a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins using high-resolution liquid chromatography tandem mass spectrometry. We demonstrate the sensitivity of our method for small tissues by acquiring data from brain, pituitary gland, and gonads of single zebrafish samples. This approach promises to shed light on the hormonal pathways and their interrelationships, providing knowledge on the integration of hormone systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping