Figure 4

Effect of combination treatment on zebrafish embryos infected with mWasabi-labelled Mmar producing Mtb BlaC variants at 4 dpi. (a) Representative larvae showing systemic infection from the control group and treated with ampicillin, avibactam, or both. Arrows indicate collections of bacteria indicative of granuloma formation, and percentages indicate the percentage of the mean of the control. (b) Bacterial load of Mmar producing either wild-type (black dots) or K234R Mtb BlaC (blue dots) as represented by fluorescence intensity after being given the indicated treatment 1 dpi. Each dot represents a larva. Larvae were injected with 1 nL of 29 mg mL⁻¹ ampicillin and 22 mg mL⁻¹ avibactam in PBS (estimated concentration 100 μg mL⁻¹ ampicillin and 75 μg mL⁻¹ avibactam in the embryo, n = 69 for WT and n = 62 for K234R) or PBS only (ctrl, n = 66 for WT and n = 63 for K234R). Data were accumulated in three independent experiments and normalized by setting the mean of the control to 100%. Error bars represent the mean and standard error. Mood’s median test was used to compare treated groups: **** = p < 0.0001.