FIGURE

Fig. 3

ID

ZDB-FIG-230920-3

Publication

Bian et al., 2023 - Genetic hyperactivation of Nrf2 causes larval lethality in Keap1a and Keap1b-double-knockout zebrafish

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Fig. 3

Upregulation of Nrf2-target genes in keap1a;keap1b-DKO larvae. (A) WISH analysis of gstp1.2 expression in 5-dpf offspring from Ka0Kb1N1 parents. The arrowhead indicates the gill. The numbers in each picture indicate the numbers of larvae with similar staining profiles and tested larvae. (B) qRT-PCR analysis of the relative expressions of gstp1.2, prdx1, txn, and gss in wild-type, Ka0Kb0N2, and Ka0Kb0N0 larvae at 5 dpf. The expression of the wild-type specimens was normalized to 1. Asterisks indicate significant differences (*p < 0.05, **p < 0.01).

Expression Data

Genes:	gss gstp1.2 prdx1 txnb
Fish:	AB keap1a^it302/it302 (AB) keap1b^it308/+; keap1a^it302/it302 (AB) keap1b^it308/it308; keap1a^it302/it302 (AB) nfe2l2a^fh318/+; keap1a^it302/it302 (AB) nfe2l2a^fh318/fh318; keap1a^it302/it302 (AB) nfe2l2a^fh318/+; keap1b^it308/+; keap1a^it302/it302 (AB) nfe2l2a^fh318/+; keap1b^it308/it308; keap1a^it302/it302 (AB) nfe2l2a^fh318/fh318; keap1b^it308/+; keap1a^it302/it302 (AB) nfe2l2a^fh318/fh318; keap1b^it308/it308; keap1a^it302/it302 (AB) (all 10)
Anatomical Terms:	gut whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	keap1b^it308/it308; keap1a^it302/it302 (AB) nfe2l2a^fh318/+; keap1b^it308/it308; keap1a^it302/it302 (AB) nfe2l2a^fh318/fh318; keap1b^it308/it308; keap1a^it302/it302 (AB)
Observed In:	gut whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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