Fig. 1.

Pigment patterns in wild-type and kcnj13 mutant D. rerio and D. aesculapii. (A-H) Pigment patterns in D. rerio wild type (A), D. aesculapii wild type (B), D. rerio kcnj13^t24ui (C), D. aesculapii kcnj13^t11mp (D), D. rerio kcnj13^txg6 (E) and D. rerio kcnj13^td15 (F). kcnj13^t24ui and kcnj13^td15 were crossed to produce trans-heterozygous kcnj13^tui24/td15 F1 fish (not shown), which were then incrossed to generate F2 fish with the genotypes kcnj13^t24ui (G; n=8) and kcnj13^td15 (H; n=12). (I-M) Melanophore clearance in kcnj13^tdxg6 is similar to wild type during the development of the first light stripe between 25 and 45 dpf (n=2). (N-O′) D. rerio wild-type (N,N′) and kcnj13^tdxg6 (O,O′) patterns at juvenile stage (J), 11 mm standard length (SL; for staging see Materials and Methods) (n>10). In the mutants, iridophores fail to reiterate the consecutive light stripes, which ultimately leads to fewer and broader stripes with occasional interruptions. Light and dark grey bars represent light and dark stripe areas, respectively. Stripes are denoted as in Parichy et al. (2009).