Fig. 1

l-CaD is expressed in PCa cell lines, and CALD1 co-expresses with positive regulators of EMT in PCa patient data sets.

A Western blot depicting l-CaD protein expression in commercial PCa cell lines. B Barplots representing CALD1 mRNA expression data in PCa cell lines acquired from Cancer Cell Line Encyclopedia. C Frequency of CALD1 alterations in publicly available PCa patient data sets containing CALD1 mutation and copy number alteration data [67–78]. D Representative Western blot depicting l-CaD expression and barplot depicting pooled densitometry of Western blot bands from three biological repeats after 72 h of 5% CSS culture and 24 h DHT treatment. E Venn diagram showing the concordance of top 1000 mRNAs co-expressed with CALD1 between PCa patient data sets available on cBioPortal. F List of concordant hits of mRNA co-expression with CALD1. G MSigDB (version 7.5) hallmark gene sets that significantly overlap and ten MSigDB GO:BP gene sets with the highest percentage of overlap with the list of mRNA concordantly co-expressed with CALD1.