Fig. 4

Figure 4. PFOA, PFHxA, and GenX suppressed the respiratory burst in vitro at 96 hr. After differentiation to nHL-60, cells were exposed to vehicle control or (A) PFOA, (B) PFHxA, (C) GenX, (D) PFOS-K, (E) PFHxS, (F) Nafion byproduct 2, (G) PFNA, (H) PFBS, or (I) PFMOAA-Na for 96 hr. Cells were then plated into a 96 well plate and stimulated with PMA to produce ROS, which was detected with DHR. Maximum fluorescence values are reported here. The entire fluorescence (AUC) values are provided in Supplemental Figure S12. Wells with no cells but with PMA and DHR, and cells receiving no PMA were included as controls. Cells treated with Bis I, a protein kinase C inhibitor, were included as a positive control for inhibition of the respiratory burst. Data shown are from three, combined, independent biological replicates, except for PFHxA, which had four biological replicates. Each biological replicate included eight technical replicates per treatment group. Individual symbols represent individual wells of a 96-well plate. Statistical significance (*p < 0.05) was determined by a one-way ANOVA with a Dunnett’s post-hoc test for pair-wise comparisons to the vehicle control.