Fig. 3

Figure 3. PFHxA and GenX suppressed the respiratory burst in vivo at 96 hrs. Zebrafish larvae were exposed for 96 hr to (A) PFOA, (B) PFHxA, (C) GenX, (D) PFOS-K, (E) PFHxS, (F) Nafion byproduct 2, (G) PFNA, (H) PFBS, or (I) PFMOAA-Na. Larvae were then washed and distributed into a 96 well-plate. Larvae were then treated with PMA, to induce ROS production, and H₂DCFDA to measure ROS produced. Fluorescence of H₂DCFDA was measured for 2.5 hr on a fluorescent plate reader set to 28.5°C. Larvae receiving no PMA and no H₂DCFDA were included as controls. Larvae treated with Bis I, a protein kinase C inhibitor, were included as a positive control for inhibition of the respiratory burst. Data shown are from at least three, combined, independent biological replicates with 4-16 larvae/treatment group. Data represent the maximum amount of fluorescence over the entire testing period, with each symbol representing an individual larvae. Statistical significance (*p < 0.05) was determined by a one-way ANOVA with a Dunnett’s post-hoc test for pair-wise comparisons to the vehicle control. AUC measures can be seen in Supplemental Figure S5. Concentration responses are provided in Supplemental Figure S6.