FIGURE 3

Retinal degeneration in rpgra^−/− zebrafish revealed by histologic analysis. (A) Schematic showing the data collected region of WT or rpgra^−/− retina. (B) Retinal sections from the dark-adapted WT and rpgra^−/− zebrafish stained with hematoxylin and eosin (H&E). The red lines indicate the thicknesses of the ONL. RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars, 50 μm. (C) Statistical results of the thickness of the retinal outer nuclear layer in each month’s zebrafish (n = 8) were analyzed using a two-tailed Student’s t-test and shown as mean ± SD. **, 0.001 < p < 0.01; ***, p < 0.001.