Fig. 7

hapln1a+ cells adjacent to coronary sprouts express the serpine1 gene.

a Visualization of EGFP⁺ cells and in situ signals of serpine1 mRNA in whole-mount view of the ventricular surface of juvenile deltaC:EGFP animals. Red dashed rectangle is enlarged. Red arrow indicates coronary sprouts and arrowheads indicate in situ signals. The serpine1 in situ signals are located laterally and ahead of EGFP⁺ signals. n = 7. Scale bar, 50 µm. b Quantification of the two types of positions: serpine1 signals within 30 µm. of coronary extensions and more than 30 µm, from experiments in a. n = 10 animals. The 69% quantified coronary extensions have serpine1 signals within 30 µm in radius, and 31% of counted coronary extensions have no serpine1 signals within 30 µm. Mann–Whitney rank-sum test (two-sided); made for two comparisons. Data are presented as mean values ± SD. Source data are provided as a Source Data file. c In situ of serpine1 mRNA in section views of adult injured deltaC:EGFP hearts. Red arrowheads indicate in situ signals. Yellow dashed lines represent surgery plane. Red dashed lines outline ventricular apex. Yellow rectangle area is enlarged in d. Red arrowheads label serpine1 in situ signals in the wound edge. n = 5. Scale bar, 50 µm. d 3-D view of serpine1 in situ signals and deltaC:EGFP⁺ cells in the wound area. Red arrow indicates the coronary tip located in the area distributed with in situ signals. Scale bar, 200 µm.