FIGURE

Fig. 2

ID

ZDB-FIG-230614-2

Publication

Grissenberger et al., 2022 - High-content drug screening in zebrafish xenografts reveals high efficacy of dual MCL-1/BCL-X_L inhibition against Ewing sarcoma

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Fig. 2

Fig. 2. Automated imaging and tumor size quantification of xenotransplanted zebrafish larvae A) Schematic representation of the imaging workflow: Xenotransplanted larvae were anesthetized, embedded in 96-well ZF plates (1 larva/well) and imaged in brightfield (BF) and fluorescence on the Operetta high-content imager (PerkinElmer). B) Representative images of shSK-E17T zebrafish xenografts imaged on consecutive days (1 dpi - 4 dpi), automated tumor detection and tumor area quantification. C) Measured tumor size areas from 1 dpi to 4 dpi (n = 15). Scale bar in B is 250 μm. Statistical analysis in C was performed with a repeated measure (RM) one-way ANOVA, ****: p ≤ 0.0001, ***: p ≤ 0.001. Error bars represent SEM of 15 individual larvae.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancer Lett.