Fig. 5.

Mutations in notch1a impact the precision of the inversion. (A) Overshooting versus final turn in the rotations of cell pairs from wild type, emx2 and notch1a mutants. We defined overshooting as the difference between the maximal turn and the final turn (absolute cumulative angle). (B) The maximal and final turns of >90° and >180° for cell pairs from wild-type, emx2 mutant and notch1a mutant larvae. The sketches below illustrate the definition of categories with an example. (C) Comparison of cell pair wobbling and final turn. Wobbling was defined as the cumulative angle changes minus final turn. (D) Noise of rotating cell pairs was calculated as the arc length of each trajectory normalized by the shortest path from starting position to final position. Statistics were calculated using the Anderson-Darling test. (A-D) n (number of cell pairs)=71 from wild-type, 42 from emx2 mutant, 22 from notch1a mutant larvae. Box plots show median values (middle bars) and first (Q1) to third (Q3) interquartile ranges (boxes); upper whisker is either 1.5× the interquartile range or the maximum value (whichever is the smallest) and lower whisker is either 1.5× the interquartile range or the minimum value (whichever is the biggest). Each point represents the cells during a rotation.