PUBLICATION
Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo
- Authors
- Kozak, E.L., Miranda-Rodríguez, J.R., Borges, A., Dierkes, K., Mineo, A., Pinto-Teixeira, F., Viader-Llargués, O., Solon, J., Chara, O., López-Schier, H.
- ID
- ZDB-PUB-230323-37
- Date
- 2023
- Source
- Development (Cambridge, England) 150(9): (Journal)
- Registered Authors
- Mineo, Alessandro
- Keywords
- Multicellular rotations, Patterning, Regeneration, Symmetry breaking, Zebrafish
- MeSH Terms
-
- Animals
- Epithelium
- Hair Cells, Auditory*
- Mechanoreceptors
- Microscopy, Video
- Zebrafish*
- PubMed
- 36946430 Full text @ Development
Citation
Kozak, E.L., Miranda-Rodríguez, J.R., Borges, A., Dierkes, K., Mineo, A., Pinto-Teixeira, F., Viader-Llargués, O., Solon, J., Chara, O., López-Schier, H. (2023) Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo. Development (Cambridge, England). 150(9):.
Abstract
Collective cell rotations are widely used during animal organogenesis. Theoretical and in vitro studies have conceptualized rotating cells as identical rigid-point objects that stochastically break symmetry to move monotonously and perpetually within an inert environment. However, it is unclear if this notion can be extrapolated to a natural context, where rotations are ephemeral and heterogeneous cellular cohorts interact with an active epithelium. In zebrafish neuromasts nascent sibling hair cells invert positions by rotating≤180° around their geometric center after acquiring different identities via Notch1a-mediated asymmetric repression of Emx2. Here we show that this multicellular rotation is a three-phasic movement that progresses via coherent homotypic coupling and heterotypic junction remodeling. We found no correlation between rotations and epithelium-wide cellular flow or anisotropic resistive forces. Moreover, the Notch/Emx2 status of the cell dyad does not determine asymmetric interactions with the surrounding epithelium. Aided by computer modeling, we suggest that initial stochastic inhomogeneities generate a metastable state that poises cells to move, spontaneous intercellular coordination of the resulting instabilities enables persistently directional rotations, whereas Notch1a-determined symmetry breaking buffers rotational noise.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping