FIGURE

Figure 3.

ID

ZDB-FIG-230410-26

Publication

Zhou et al., 2023 - Assessment of Nonalcoholic Fatty Liver Disease Symptoms and Gut-Liver Axis Status in Zebrafish after Exposure to Polystyrene Microplastics and Oxytetracycline, Alone and in Combination

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Figure 3.

(A) Expression levels of inflammation-related genes and (B) western blot of TNF-α in zebrafish livers after 28-d exposure to control, MPs, OTC, and MP-OTC, respectively (n=3 for each experimental group for both gene expression and western blot analyses). Gene expressions were determined by real-time PCR with a Bio-Rad real-time system (CFX96; Bio-Rad) and the relative TNF-α expression levels were quantified using ImageJ. The corresponding numeric data are provided in Table S6. Data (means±SEs) with different superscripts above were significantly different at p<0.05 (one-way analysis of variance and Tukey’s post hoc tests). Note: MDA, malondialdehyde; MP-OTC, microplastics and oxytetracycline; MPs, microplastics; OTC, oxytetracycline; PCR, polymerase chain reaction; ROS, reactive oxygen species; SE, standard error; TNF-α, tumor necrosis factor-α.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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