FIGURE

Figure 1

ID

ZDB-FIG-230401-3

Publication

Pezzotta et al., 2023 - Combined Inhibition of Hedgehog and HDAC6: In Vitro and In Vivo Studies Reveal a New Role for Lysosomal Stress in Reducing Glioblastoma Cell Viability

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Hh and HDAC6 inhibition impaired U87-MG cell viability. (A) MTT assay of U87-MG cells treated for 48 h with vehicle (CT), 8 μM of TubA (T8), 10 μM of cyclo (C10) and 8 μM of TubA + 10 μM cyclo (T8C10). (B–E) Representative images of the head region of 3 dpf zebrafish embryos xenotransplanted with labeled U87-MG cells pretreated with (B) CT, (C) T8, (D) C10, and (E) T8C10. (F) Quantification of the tumor area at t1 (24 h postinjection, hpi) normalized to the tumor area at t0 (immediately after U87 injection). (G) Western blot analyses and quantification of pERK and pAKT protein expression levels following 48 h treatments with or without T8 and C10, alone or in combination. Data are expressed as the percentage of the control (A,G) or mean ± standard deviation (F). CT—vehicle; TubA/T—tubastatin A; cyclo/C—cyclopamine. One-way ANOVA with Tukey post hoc correction. *** p < 0.001; ** p < 0.01; * p < 0.05; nonsignificant data are not shown. Scale bare indicates 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.