FIGURE
Fig. 6
- ID
- ZDB-FIG-230328-29
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- Zheng et al., 2022 - A Simple, Robust, and Cost-effective Method for Genotyping Small-scale Mutations
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Fig. 6
Genotyping wild-type, heterozygous, and homozygous mice with a single nucleotide substitution in cfh.
(a) The expected DNA sequences of the wild-type (wt) and mutant (mu) murine cfh. (b–c) The Surveyor nuclease digestion patterns of various PCR products in the absence (−) and the presence (+) of a wt sequence, respectively. Here, * and # indicate the presence of the cleavage bands (arrowheads) in the absence (i.e., heterozygous) and the presence (i.e., homozygous) of a wt PCR sequence, respectively. (d–e) Sanger sequencing confirmed the wt and mu (one nucleotide substitution) sequences revealed above. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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