Image
|
Figure Caption
Fig. 6
Genotyping wild-type, heterozygous, and homozygous mice with a single nucleotide substitution in cfh.
(a) The expected DNA sequences of the wild-type (wt) and mutant (mu) murine cfh. (b–c) The Surveyor nuclease digestion patterns of various PCR products in the absence (−) and the presence (+) of a wt sequence, respectively. Here, * and # indicate the presence of the cleavage bands (arrowheads) in the absence (i.e., heterozygous) and the presence (i.e., homozygous) of a wt PCR sequence, respectively. (d–e) Sanger sequencing confirmed the wt and mu (one nucleotide substitution) sequences revealed above.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ J Clin Transl Pathol