FIGURE

Fig. 4

ID

ZDB-FIG-230203-15

Publication

Mamot et al., 2021 - Ethylenediamine derivatives efficiently react with oxidized RNA 3' ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Application of dually labelled RNA probes for enzymatic activity monitoring. (A) Sequence and theoretical secondary structures of Cy5-RNA₃₅-Cy3, Cy5-m⁷GRNA₃₅-Cy3 and Cy5-m⁷GRNA₂₇₆-Cy3 probes and illustration of studied DNA sequences complementary to regions of the probes. (B) Time-dependent changes of emission spectra and Cy3/Cy5 fluorescence intensity ratio after addition of RNase A, RiboLock and RNase A, RNase T1, RNase R or Dcp1/2 to Cy5-RNA₃₅-Cy3 or Cy5-m⁷GRNA₃₅-Cy3 probes. (C) Time-dependent changes of emission spectra, PAGE of reaction products, and Cy3/Cy5 fluorescence intensity ratio after addition of RNase H to probe (Cy5-RNA₃₅-Cy3 or Cy5-m⁷GRNA₂₇₆-Cy3) or DNA-probe duplex.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.