FIGURE
Fig. 2
- ID
- ZDB-FIG-230203-13
- Publication
- Mamot et al., 2021 - Ethylenediamine derivatives efficiently react with oxidized RNA 3' ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation
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Fig. 2
One-pot 3’-end RNA labelling. (A) Reaction scheme. Conditions: i) pU3 (140 µM), aqueous NaIO4 (1.4 mM), 30 min, and 25°C in the dark. ii) Addition of R-EDA (1.0 mM) and NaBH3CN (20 mM) in KH2PO4 buffer (0.1 M pH 6.0). HPLC-determined yields after two hours of reductive amination are presented for each N-nucleophile. (B) HPLC chromatograms of reaction with Cy3-EDA at different stages. (C) Second-order reaction rate constant k values determined for reductive amination reaction between pU3-dial (100 µM) and different N-nucleophiles (1 mM). |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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