Fig. 1
- ID
- ZDB-FIG-230120-2
- Publication
- Adane et al., 2021 - STAG2 loss rewires oncogenic and developmental programs to promote metastasis in Ewing sarcoma
- Other Figures
- All Figure Page
- Back to All Figure Page
Loss of STAG2 does not consistently alter cell growth but changes the composition of the cohesin complex and renders cells sensitive to STAG1 deletion (A) Hockey plots depicting the distribution of STAG2, SMC1A, SMC3, and RAD21 gene effect (CERES) scores across the 789 cell lines in CRISPR (Avana) Depmap v20Q3 data. (B) Line graphs showing mean ± SD of cumulative doublings for parental or clonally selected non-targeting (NT) or STAG2 KO A673 and TC71 cells. Pairwise comparative analysis for exponential growth fitted curves. Extra-sum-of-squares F test, ∗∗p < 0.01; ns, not significant. (C) EdU (5-ethynyl-2′-deoxyuridine) incorporation-based cell-cycle profiling and representative flow-cytometry plots (left) are shown. Two independent experiments as mean ± SD bar plots (right). Two-way ANOVA; ns, not significant. (D and E) Subcellular fractionation (D) or immunoprecipitation with SMC1A, SMC3, or IgG (E) followed by western blot was performed for the indicated proteins. (F) Genome-scale CRISPR/Cas9 screen in isogenic NT and STAG2 KO A673 clonal cells. Shown is the volcano plot for gene effect size versus −log10(p value) for the genome-wide differential analysis of isogenic NT versus STAG2 KO processed reads; dots represent genes (limma eBayes for MAGeCK gene effect scores, effect size ≤ −0.3, adjusted p ≤ 0.10). |
Reprinted from Cancer Cell, 39, Adane, B., Alexe, G., Seong, B.K.A., Lu, D., Hwang, E.E., Hnisz, D., Lareau, C.A., Ross, L., Lin, S., Dela Cruz, F.S., Richardson, M., Weintraub, A.S., Wang, S., Iniguez, A.B., Dharia, N.V., Conway, A.S., Robichaud, A.L., Tanenbaum, B., Krill-Burger, J.M., Vazquez, F., Schenone, M., Berman, J.N., Kung, A.L., Carr, S.A., Aryee, M.J., Young, R.A., Crompton, B.D., Stegmaier, K., STAG2 loss rewires oncogenic and developmental programs to promote metastasis in Ewing sarcoma, 827-844.e10, Copyright (2021) with permission from Elsevier. Full text @ Cancer Cell