Figure 5

Overexpression of Anp32a could promote cell proliferation in SCI embryos during recovery. (A) The experimental flowchart. Anp32a mRNA was injected into embryos from zebrafish transgenic line Tg(mnx1:GFP) at the one-cell stage, followed by SCI at 48 hpf and staining with anti-PH3 at 18 and 24 hpi. (B) Proliferation patterns around the lesion site of embryos treated as indicated at 18 and 24 hpi were presented by immunofluorescence through antibody against phosphorylated histone H3 (PH3; Red) and visualized by laser confocal microscopy. Arrow indicates the SCI site, while white dotted box indicates the calculation area. (C) Statistical count of PH3-positive cells between SCI-Anp32a-overexpressed embryos (n = 11 at 18 hpi; n = 10 at 24 hpi) and SCI embryos without overexpression of Anp32a (n = 11 at 18 hpi; n = 10 at 24 hpi). A) Scale bar: 100 μm. The Student’s t-test was used to perform statistical analysis (*, p < 0.05; p < 0.01; ***, p < 0.001; error bars indicate mean ± SD).