FIGURE

Fig. 4

ID

ZDB-FIG-221212-34

Publication

Bernardello et al., 2021 - Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy

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Fig. 4

Imaging of YFP-GR in a zebrafish embryo. (A) Maximum intensity projection of a z-stack obtained using LSFM imaging of a developing 1dpf Tg(actb2:mCherry-H2A) zebrafish embryo injected with a YFP-GR expression vector (only H2A signal shown in A). Scale bar 300 µm. (B/C). Higher magnification image of the highlighted ROI showing the (B) mCherry-H2A and (C) the YFP-GR signal (maximum intensity projections, scale bars 50 µm). Imaging performed using a different LSFM setup. The expression of GR is evident, as well as the localization within the eYSN. (D) Image of a nuclei in the yolk from a 2dpf live zebrafish embryo obtained using the LSFSMM setup. Only H2A signal shown, scale bar 10 µm. (E-G) Three individual YFP-GR molecules imaged through the LSFSMM setup within the ROIs highlighted in D (white squares), in a 2 dpf zebrafish embryo. Scale bars 2 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Opt. Express