PUBLICATION
Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy
- Authors
- Bernardello, M., Gora, R.J., Van Hage, P., Castro-Olvera, G., Gualda, E.J., Schaaf, M.J.M., Loza-Alvarez, P.
- ID
- ZDB-PUB-211109-12
- Date
- 2021
- Source
- Biomedical Optics Express 12: 6205-6227 (Journal)
- Registered Authors
- Bernardello, Matteo, Gualda, Emilio, Loza-Alvarez, Pablo, Schaaf, Marcel J. M.
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 34745730 Full text @ Biomed. Opt. Express
Citation
Bernardello, M., Gora, R.J., Van Hage, P., Castro-Olvera, G., Gualda, E.J., Schaaf, M.J.M., Loza-Alvarez, P. (2021) Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy. Biomedical Optics Express. 12:6205-6227.
Abstract
Single-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet fluorescence microscopy setup is presented for imaging individual proteins inside living zebrafish embryos. The optical configuration makes this design accessible to many laboratories and a dedicated sample-mounting system ensures sample viability and mounting flexibility. Using this setup, we have analyzed the dynamics of individual glucocorticoid receptors, which demonstrates that this approach creates multiple possibilities for the analysis of intracellular protein dynamics in intact living organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping