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Fig. 2

ID

ZDB-FIG-221020-21

Publication

Kuhn et al., 2022 - Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model

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Fig. 2

Localization of morphogens in cell-cell interfaces and extracellular cavities.

a Top: Overlay of the memGFP signal, the manually curated ROI separating cell-cell-interfaces (blue, I) and extracellular cavities (red, C); tracks assigned to interfaces (blue), cavities (red) and not assigned (green) are also shown. Bottom: Tracking-and-localization-microscopy (TALM) image showing the total number of localizations over 1000 frames or 11.7 s in each 2 × 2 pixel bin; scale bar: 5 µm. b Ratios of localization densities in cavities and interfaces calculated for each movie (for statistics see Supplementary Table ⁵). Values above 10 are not displayed (3.4% of all movies). p-values (Cyclops - Squint, Cyclops - Lefty1, Cyclops - Lefty2, Squint - Lefty1, Squint - Lefty2: <0.001; Lefty1 - Lefty2, Lefty1 - secHalo, Lefty2 -secHalo: >0.999) were calculated using the Kruskal-Wallis-Test. Solid black lines indicate the median values, dashed black lines the 0.25 and 0.75 quantiles. Source data are provided as a Source Data file for Fig. 2b.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.