FIGURE 2

Both FL and ∆VR isoforms of mouse Eomes are functionally equivalent to zebrafish Eomesa in the early embryo. WISH analysis of ectoderm markers vgll4l and zic3 in mid/late blastulas (4 h.p.f.) embryos (A,B), organiser markers noto and chrd(C,D) in early gastrulas (6 h.p.f.), or DFC markers sox17, vgll4l and foxj1a(E–H) in early/mid gastrulas (6.5 h.p.f.). Embryos have been injected at the 1 cell stage to overexpress either mouse EomesFL, Eomes∆VR or zebrafish Eomesa. N = 2. Total numbers of embryos scored per condition are indicated. Representative images of expression patterns per gene per category are shown. (A–D) Animal views; dorsal to the right. (E) Animal views; dorsal to the right. (F–H) Vegetal views; dorsal to the right. Panel G indicates the percentage of embryos with greater intensity of dorsal foxj1a WISH staining, while panel H indicates percentages of embryos with ectopic foxj1a staining. Type I–wild type expression; Type II–ectopic dorsolateral expression with clear primary dorsal DFC cluster; Type III–dorsolateral expression with no defined primary cluster; Type IV–ectopic expression in the ventral margin. Dotted ovals indicate primary DFC clusters. Arrowheads indicate ectopic DFC marker expression.