FIGURE

FIGURE 7

ID

ZDB-FIG-220921-65

Publication

Field et al., 2022 - Involvement of transmembrane protein 184a during angiogenesis in zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 7

KD of Tmem184a results in decreased levels of VE-cadherin. Embryos were injected with control or ATG-MO for Tmem184a, sorted for GFP positive and fixed at 43 hpf. Embryos were stained with the Anti-zebrafish VE-cadherin antibody as described in Methods and imaged on the Zeiss LSM 880 confocal microscope at 20x. (A) Sections in the mid-section of representative embryos are illustrated. Sections illustrated are maximum intensity images as in Methods. Data from 34 Tmem184a KD ISVs and 29 control ISVs from four embryos of each type were analyzed for VE-cadherin intensity across all ISVs in the mid-sections imaged and a graph illustrating relative VE-cadherin is shown in (B). A student’s T test was used to determine significance, p < 0.001 (C) Western blots of identically treated embryo lysates are shown.

Expression Data

Antibody:	Ab14-cdh5
Fish:	y1Tg y1Tg + MO1-tmem184a
Knockdown Reagent:	MO1-tmem184a
Anatomical Terms:	intersegmental vessel whole organism
Stage:	High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg y1Tg + MO1-tmem184a
Knockdown Reagent:	MO1-tmem184a
Observed In:	intersegmental vessel whole organism
Stage:	High-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.