FIGURE

FIGURE 6

ID

ZDB-FIG-220921-64

Publication

Field et al., 2022 - Involvement of transmembrane protein 184a during angiogenesis in zebrafish embryos

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FIGURE 6

Tmem184a KD Causes an Increase in Cell Proliferation. (A) Example images of PH3 staining in SC, Tmem184a, and Vegfr2 MO knockdown embryos at 48 hpf. White arrows indicate where the PH3 staining co-localizes with the GFP in the vasculature of the ATG MO-treated embryo, highlighted in yellow. Z-stack images were rotated to confirm nuclear PH3 in the EC. Embryos injected with the Tmem184a MO (n = 21) exhibit an increase in the percentage of ISVs that contain an PH3 stained nucleus compared to either SC (n = 21) or Vegfr2 (n = 11) injected embryos. (B) One PH3 nucleus in an ISV is shown in all planes. Cross-hairs intersect at the stained ISV localized nucleus. (C) The graph illustrates the percentage of ISVs per embryo containing an PH3-stained nucleus. p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO1-tmem184a y1Tg + MO2-kdrl
Knockdown Reagents:	MO1-tmem184a MO2-kdrl
Observed In:	intersegmental vessel endothelial cell proliferation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.