Fig. 2

(A) Schematic representation of glucose metabolism. (B) OB gene expression profile of glycolytic enzymes (green), ldha (magenta) and OXPHOS components (blue) at 6 hpa relative to uninjured conditions (0 hpa) obtained from the OB ArrayXS. The horizontal axis represents the log2 fold-change and p-values on a negative log10 scale. Statistical analysis with t test and Welch’s correction (n=3 biological replicates), Mean ± SD are displayed. (C, D) Relative gene expression of glycolytic enzymes (green), ldha (magenta) and OxPhos components (blue), in the whole fin stump, at (C) 6 hpa and at (D) 24 hpa in comparison to uninjured conditions (0 hpa). Statistical analysis with paired t test (n=5 (C) and 4 (D) biological replicates). (E) Metabolite measurements at 6 hpa (clean columns) and 24 hpa (stroked columns) in relation to uninjured conditions (0 hpa), in the whole fin stump. Statistical analysis with Mann-Whitney test (n=4 biological replicates). (F–K) Live imaging of 2NDBG uptake (green) in osx:mCherry fish (magenta) at (F–H) 0 hpa and (I–K) 24 hpa. Arrows indicate uptake of 2NBDG in the intersegment regions. White dashed line delineates the regenerated tissue. Scale bar represents 500 µm. (L–M) Intensity of 2NBDG uptake in regions close and distant to the amputation site, at (L) 0 hpa and (M) 24 hpa. Red * indicate peaks of 2NDBG uptake in the intersegments. (N) Ratio of 2NBDG uptake at 0 hpa and 24 hpa. Statistical analysis on graph corresponds to Mann-Whitney test. Mean ± SD are displayed (n=54 and 83 bony-rays). ns: not significative; *p<0,0001. See Figure 2—source data 1.