|
Most APAP-treated embryos express collagen type II, a marker for differentiated cartilage, in the craniofacial region. (A–C) Ventral views of 72-hpf embryos processed for col2a1 (collagen type II) IHC. Untreated embryos (A) express col2a1 in all craniofacial cartilages. In most APAP-treated embryos (B), col2a1 expression is equally robust but reflects the absence of numerous cartilage types. Diffuse col2a1 expression is consistently observed around the developing ears and at the base of the head. In other APAP-treated embryos (C), col2a1 expression is completely absent in the anterior head region. The numbers of col2a1-labeled, APAP-treated embryos are labeled in parentheses. Abbreviations: ceratobranchial cartilages, cb; ventral ceratohyal, ch; ethmoid plate, ep; dorsal hyosymplectic, dh; Meckel’s cartilage, mc; palatoquadrate, pq. Scale bar (in (A)) for (A–C), 200 µm.
|
