Figure 1

Both z-Idua enzymatic activity and the accumulation of heparan sulfate (HS) in zebrafish embryos were closely correlated with knockdown or overexpression of z-idua mRNA injected into zebrafish embryos. (A) Zebrafish embryos at one-cell stage were injected with different doses of z-idua-MO, as indicated, followed by detection of z-Idua enzymatic activity at 24 hpf. The z-Idua enzymatic activity of the untreated control group was normalized as 1 for comparison with embryos injected with different doses of z-idua-MO. (B) The z-Idua enzymatic activity of zebrafish embryos injected with different doses of z-idua mRNA, as indicated. The z-Idua enzymatic activity of the untreated control group was normalized as 1 for comparison with the embryos injected with different doses of z-idua mRNA. (C) HS concentration was detected at 120 hpf of zebrafish embryos injected with 12 ng of z-idua-MO. The HS concentration of the untreated control group was normalized as 1 for comparison with the z-idua-MO-injected embryos. One trial experiment was carried out for 40 larvae. Data of each group were averaged from three independent experiments. Student’s t-test was used to determine significant differences between the two groups (**, p < 0.01).