Figure 2

(a-c) In vivo epithelial injury-induced proliferation in response to naphthalene was analysed by EdU incorporation of CD45^-/CD31^-/EpCAM⁺ cells (n=3-8, analysed by one-way ANOVA with Dunnett's multiple comparisons test). (d,e) Injury-induced epithelial proliferation was measured in Panx1+^/+ and Panx1^-/- mice, with naïve mouse data shown for comparison (n=3-6 naïve, n=5-8 post-naphthalene, two independent experiments, assessed by t-test). (f) Zebrafish at 3 days post-fertilisation were microinjected with 1nL of the Panx1 pharmacological inhibitors trovafloxacin (80mM) or spironolactone (120mM), or DMSO control, and tailfins were transected. (g,h) Tailfin regeneration rate was assessed at 48h (n=20-22 separate animals, three separate experiments, assessed by one-way ANOVA with Holm-Šídák's multiple comparisons test). Scale bar, 100um. (i) Proliferation in the regenerating tailfin analysed by EdU incorporation, imaged by light sheet fluorescent microscopy (n=15-21 per group, assessed by Holm-Šídák's multiple comparisons test). (j) Similarly, Panx1a morphants (or control sequence morphants) underwent tailfin transection and regeneration rate was assessed at 48h post-injury (n=13 separate animals, two separate experiments, assessed by t-test), with (k) tailfin proliferation analysed by EdU incorporation, (n=21 per group, one experiment, assessed by t-test). *p<0.05, ***p<0.001, **** p<0.0001.