FIGURE 1

A high throughput analysis of 5 stages of oocyte development. (A) Unsupervised hierarchical clustering of all genes that were changed between any of the stages. Samples are ordered according to clustering. (B) PCA of the duplicates of the different stages. (C) Control gene expression confirms the accuracy of our data. Germ cell specific genes are expressed, but somatic markers show background expression comparable with expression of non-ovarian genes images of oocyte stages groups were modified from Elkouby and Mullins (Elkouby and Mullins 2017a). (D) Heat map of k-means clustering of genes that were differentially expressed as determined by a likelihood ratio test. Five clusters were determined to allow visualization of the expression trends. Each stage is represented by a color throughout the figures: red for Symbrk, blue for Nuc, green for MatBb, purple for Stage II, and orange for Stage III. Red denotes higher expressed genes and blue denotes lower expressed genes. The numbers are normalized relative expression. Each column is the average of the duplicates.