FIGURE

Fig. 2

ID

ZDB-FIG-220603-22

Publication

Tanaka et al., 2022 - Chemogenetic Depletion of Hypophysiotropic GnRH Neurons Does Not Affect Fertility in Mature Female Zebrafish

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Fig. 2

GnRH3 neuronal projections are reduced at 28 days of treatment in the pituitary of GnRH3 neuron-ablated females. (A) Confocal images of immunostaining with GnRH3-associated peptide antibody in the whole pituitary (posterior to top left), showing GnRH3 neuronal projections (green). Scale bars, 1 mm. The bottom panel of Tg-Mtz, an image with enhanced signals with levels and tone curve adjustments to demonstrate that GnRH3 is still detectable in the pituitary. (B) LH levels in plasma (n = 9–14). WT-Mtz, WT siblings treated with 2 mM metronidazole (Mtz); Tg-Cont, Tg(gnrh3:Gal4ff; UAS:nfsb-mCherry) treated with vehicle; Tg-Mtz, Tg(gnrh3:Gal4ff; UAS:nfsb-mCherry) treated with 2 mM Mtz. p > 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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