Fig. 3

Figure 3. Zebrafish larvae undergo alteration of their swimming kinematic during seizures. (a) Scheme representing the behavioral tracking protocol adopted. The larvae were tracked along 5 min before the exposure to PTZ and 30–60 min after. (b) Swimming trajectories over 5 min of behavioral recording of one larva for each of the following conditions: pre-exposure (CTRL) and 60 min after the exposure to the different PTZ concentrations. (c,d) Average total distance traveled (c) and percentage of time spent in movement (d) in 5 min recording in the 3 different tracking windows (CTRL, 30 and 60 min), in larvae exposed to one of the four PTZ concentrations tested (see legend for colors). (e) Representative swimming bout shapes observed in each of the conditions reported, presented as larval displacement over time. Traces are color-mapped according to swimming velocity (warmer colors higher speed). (f–j) Average kinematic features of swimming bouts -maximum speed (f), maximum acceleration (g), duration (h), displacement (i) and frequency (j)—during 5-min recording epochs before PTZ exposure and 30–60 min after. * indicates p-value < 0.05 for intra-group comparison with respect to control (CTRL) values, one-way repeated measures ANOVA and post hoc Tukey’s test (for p-values see Table S6). Diamond symbol indicates p-value < 0.05 for inter-group comparison at 60 min exposure, two-way repeated measures ANOVA and post hoc Tukey’s test (for p-values see Table S7). Color couples in each symbol indicate groups compared. Values represent mean ± s.e.m. of n = 4 larvae per concentration.