Fig. 1

Figure 1. Zebrafish brain regions present different susceptibility to convulsant effects. (a) Dorsal (upper) and lateral (lower) schemes representing in different colors the ten brain districts into which volumetric calcium imaging data were segmented for further analysis. Dotted lines show the outer larval boundaries. A: anterior; P: posterior; R: right; L: left; D: dorsal; V: ventral. (b) Scheme representing the brain functional imaging protocol adopted. After PTZ treatment, larvae were imaged for 5 min every 10, along 60 min. (c) Map showing in color-code the neuronal activity (ΔF/F₀) of one larva over time of each of the ten brain regions (colored as in panel (a)) along 60 min of seizure mapping. Warmer colors indicate higher activity. Below the map, the different brain activity regimes, discussed in Figure 2, are reported. (d) Plot showing for each of the ten brain regions (abbreviations and colors as in panel (c)) the frequency of activity peaks at different PTZ concentrations (1.0, 2.5, 7.5 and 15 mM, see legend for colors) over 60 min of activity monitoring. Values represent mean ± s.e.m. of n = 3 larvae per concentration. * indicates p-value < 0.05 for intra-group comparison with respect to control (−5 min) values, one-way repeated measures ANOVA and post hoc Tukey’s test (see Table S1 for p-values). Diamond symbol indicates p-value < 0.05 for inter-group comparison at the same time point, two-way repeated measures ANOVA and post hoc Tukey’s test (Table S2). Color couples in each symbol denote compared groups.