The expression pattern of phospho-histone-H3 in dorsal fin primordium. (a–f) Expression pattern of phospho-histone-H3 in the reducing LMFF area at 4.5–5.0 mm SL (a,b), 5.0–5.5 mm SL (c,d), and 5.5–6.0 mm SL (e–f). The lower panels (b,d,f) are magnified views of the dashed rectangles in the upper panels (a,c,e), respectively. (g,h’’) The expression pattern of UAS:EGFP of the gt1116A line and phospho-histone-H3 in dorsal fin primordium at 5.5–6.0 mm SL. Black lines in (g) indicate levels of the section shown in (h–h’’). Arrowheads in (h,h”) indicate phospho-histon-H3 signals. (i) Boxplots of phospho-histone-H3-positive cells in dorsal fin development along with the growth of the body. In (i), more than half of the 4.5–5.0 mm SL zebrafish samples, phospho-histone-H3-positive cells were not detect. Thus, we conducted statistical analysis only between 5.0–5.5 mm SL and 5.5–6.0 mm SL. (j) Boxplots of phospho-histone-H3-positive cells in dorsal fin development under SU5402 treatment. The proportions in (i) and (j) were calculated from the number of pH3-positive cells in dorsal fin primordium. Whiskers in (i) and (j) show maximum and minimum values within 1.5 times the interquartile range. Boxes show the median and 25th and 75th percentiles. The P value in (i) and (j) is the result of Brunner-Munzel test (P = 0.009701) and the result of Welch's t test (P = 0.2272), respectively. Scale bars in (a–f) and those in (h) indicate 200 μm and 50 μm, respectively.
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