ZFIN Figure: Zhang et al., 2020, Fig. 3

ZFIN ID: ZDB-FIG-220503-3

ZFIN ID: ZDB-FIG-220503-3

Zhang et al., 2020 - Copper Stress Induces Zebrafish Central Neural System Myelin Defects via WNT/NOTCH-hoxb5b Signaling and pou3f1/fam168a/fam168b DNA Methylation. Biochimica et biophysica acta. Gene regulatory mechanisms 1863(10):194612 Full text @ BBA Gene Regulatory Mechanisms

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	DsRed2 hoxb5b mbpa plp1a
Fish:	WT vu19Tg
Conditions:	chemical treatment by environment: copper(2+) chemical treatment by environment: copper(2+)
Anatomical Terms:	central nervous system trunk
Stage Range:	Prim-5 to Day 4

PHENOTYPE:

Fish:	WT vu19Tg
Conditions:	chemical treatment by environment: copper(2+) chemical treatment by environment: copper(2+)
Observed In:	central nervous system central nervous system axon trunk
Stage Range:	Prim-5 to Day 4

Fig. 3 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
DsRed2	vu19Tg	standard conditions	Long-pec	central nervous system	RTPCR
	vu19Tg	chemical treatment by environment: copper(2+)	Long-pec	central nervous system	RTPCR
	vu19Tg	chemical treatment by environment: copper(2+), chemical treatment by environment: 6-bromoindirubin-3'-oxime	Long-pec	central nervous system	RTPCR
hoxb5b	WT	standard conditions	Prim-5	trunk	ISH
	WT	chemical treatment by environment: copper(2+)	Prim-5	trunk	ISH
	WT	chemical treatment by environment: copper(2+), chemical treatment by environment: 6-bromoindirubin-3'-oxime	Prim-5	trunk	ISH
mbpa	WT	standard conditions	Day 4	central nervous system	ISH
	WT	chemical treatment by environment: copper(2+)	Day 4	central nervous system	ISH
	WT	chemical treatment by environment: copper(2+), chemical treatment by environment: 6-bromoindirubin-3'-oxime	Day 4	central nervous system	ISH
plp1a	WT	standard conditions	Day 4	central nervous system	ISH
	WT	chemical treatment by environment: copper(2+)	Day 4	central nervous system	ISH
	WT	chemical treatment by environment: copper(2+), chemical treatment by environment: 6-bromoindirubin-3'-oxime	Day 4	central nervous system	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT	chemical treatment by environment: copper(2+)	Prim-5	trunk hoxb5b expression decreased amount, abnormal
		Day 4	central nervous system mbpa expression decreased amount, abnormal
		Day 4	central nervous system plp1a expression decreased amount, abnormal
WT	chemical treatment by environment: copper(2+), chemical treatment by environment: 6-bromoindirubin-3'-oxime	Prim-5	trunk hoxb5b expression amount, ameliorated
		Day 4	central nervous system mbpa expression amount, ameliorated
		Day 4	central nervous system plp1a expression amount, ameliorated
vu19Tg	chemical treatment by environment: copper(2+)	Long-pec	central nervous system axon decreased length, abnormal
		Long-pec	central nervous system DsRed2 expression decreased amount, abnormal
vu19Tg	chemical treatment by environment: copper(2+), chemical treatment by environment: 6-bromoindirubin-3'-oxime	Long-pec	central nervous system axon decreased length, ameliorated
		Long-pec	central nervous system DsRed2 expression amount, ameliorated

Full text @ BBA Gene Regulatory Mechanisms