FIGURE

Fig. 6.

ID

ZDB-FIG-220331-7

Publication

Rosa et al., 2021 - Wasp controls oriented migration of endothelial cells to achieve functional vascular patterning

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6.

WASp controls migration and PECAM1 localization in HUVECs. (A) Phase contrast images of control and was siRNA-treated HUVECs in a confluent monolayer. (B) Scratch wound assay for control and was siRNA-treated HUVECs at baseline (immediately after removing barrier) and after16 h. (C) Quantification of wound closure at 16 h. Data are mean±s.d. of three independent experiments (six biological replicates). (D) Effective velocity of HUVECs during wound closure assay. Samples have a significant velocity difference, P<0.0001 (Welch's two sample t-test). (E) Rosette graph showing the prevalent cell direction during wound closure in control and was siRNA-treated cells. Comparison between graphs: P<0.001 (Watson's two-sample test of homogeneity). (F) HUVECs treated for control and was siRNA stained for f-actin (Phalloidin, red in combined), PECAM1 (green in combined) and nuclei (DAPI, blue).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development