FIGURE

Fig. 11

ID

ZDB-FIG-211102-25

Publication

Lee et al., 2021 - Poly(U)-specific endoribonuclease ENDOU promotes translation of human CHOP mRNA by releasing uORF element-mediated inhibition

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Fig. 11

During normal conditions, the translation of CHOP protein is blocked by huORFchop-TI due to ribosomes stalled at 81–90 nts within the 105-nt huORFchop transcript. However, Endouc/ENDOU is quickly produced when cells encounter environmental stress, e.g., heat-shock and hypoxia as in the present work. It binds to the hairpin structure of huORFchop transcript formed at 72–91 nts and cleaves it at the 80G-81U site. This endonuclease activity changes the conformation of inhibitory structure formed by huORFchop transcript, in turn, releasing the stalled ribosomes to scan through the huORFchop transcript and reinitiate at AUG of the main downstream coding sequence (DCS) of huORFchop transcript. Finally, CHOP transcript is continuously translated via the IRES activity driven by huORFchop−69-105-nt and the increase of p-eIF2α induced by Endouc/ENDOU.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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