FIGURE

Fig. 6

ID

ZDB-FIG-211009-6

Publication

Minhas et al., 2021 - Transcriptome profile of the sinoatrial ring reveals conserved and novel genetic programs of the zebrafish pacemaker

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Fig. 6

Morpholino mediated knockdown of three SAR-overexpressed candidate genes. A-H Heart morphology of control larvae and injected with 2 and 4 ng gene-specific morpholino against pard6a, prom2, and atp1a1a.2 after 72 hpf. Red arrows indicate pericardial edema, yellow arrows indicate a failure in heart looping. I-J Percentage of morphological defects observed as a result of 2 and 4 ng of MO injections, showing dose-dependent effect. Scale bar: 50 μm. K Distribution of average heartbeat rate (in beats per minute, bpm) assayed in 72 hpf larvae as a result of knockdown with 2 ng or 4 ng of MO against pard6a, prom2, and atp1a1a.2. “*” indicates that the difference in heartbeat rate compared to that of control (uninjected) group was statistically significant. Table shows average heartbeat rate and Student’s T-tested p-value

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL + MO2-pard6a AB/TL + MO3-atp1a1a.2 AB/TL + MO4-prom2
Knockdown Reagents:	MO2-pard6a MO3-atp1a1a.2 MO4-prom2
Observed In:	heart heart contraction heart looping pericardium
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Genomics