FIGURE

Figure 4

ID

ZDB-FIG-210922-30

Publication

Katz et al., 2021 - Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT

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Figure 4

Silver staining and X-ray microtomography (micro-CT) of mutant zebrafish enables comparison of melanin content and organization with wild-type larvae.

Volume renderings (A, D, G), segmented volumes (B, E, H), and representative single slices of micro-CT reconstructions through the retinal pigment epithelium (RPE; C, F, I) of representative 5 days post-fertilization (dpf) wild-type (A–C), slc24a5^b1/b1 (golden; D–E), and mitfa^w2/w2 (nacre or casper; G–I) larvae. In the segmented volumes, red = dorsal stripe, yellow = ventral stripe, green = yolk sac stripe, cyan = lateral stripes, white = RPE, gray = other body melanin, purple = lens. As compared to wild-type larvae, slc24a5^b1/b1 mutant larvae exhibit a reduction in staining throughout the body and eyes (D) but retain the overall organization of pigment layers (E). The RPE in slc24a5^b1/b1 larvae (F) is thinner than the wild-type RPE (C) with less intense staining. The mitfa^w2/w2 mutant lacks all body pigment; staining is observed only in the RPE and some argentaffin material posterior to the eyes (G–H, arrow). The RPE of the mitfa^w2/w2 mutant (I) is of similar thickness and staining intensity as the wild-type RPE (C). For each comparison, visualization settings were kept constant for the wild-type and mutant fish. § = head segment only shown. All stained samples analyzed in this study are shown in Figure 4—figure supplement 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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