Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
- Katz, S.R., Yakovlev, M.A., Vanselow, D.J., Ding, Y., Lin, A.Y., Parkinson, D.Y., Wang, Y., Canfield, V.A., Ang, K.C., Cheng, K.C.
- eLIFE 10: (Journal)
- Registered Authors
- Canfield, Victor, Cheng, Keith C.
- 3D imaging, cell biology, developmental biology, melanin, micro-CT, pigmentation, silver staining, whole-organism phenomics, zebrafish
- MeSH Terms
- Imaging, Three-Dimensional/methods*
- Silver Staining/methods*
- X-Ray Microtomography/methods*
- Zebrafish Proteins*/analysis
- Zebrafish Proteins*/chemistry
- 34528510 Full text @ Elife
Katz, S.R., Yakovlev, M.A., Vanselow, D.J., Ding, Y., Lin, A.Y., Parkinson, D.Y., Wang, Y., Canfield, V.A., Ang, K.C., Cheng, K.C. (2021) Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT. eLIFE. 10:.
We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes