FIGURE

Figure 3

ID

ZDB-FIG-210727-20

Publication

Steeman et al., 2021 - Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development

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Figure 3

Measurement of miR-145 expression with stem–loop RT-qPCR. (a) MiR-145 temporal expression levels during zebrafish development (±SEM, n = 3). (b,c) Schematic representation of sox9a (ENSDARG00000003293) and sox9b (ENSDARG00000043293) 3′UTR. Putative miRNA binding sites are shown below each region. MiR-145 sites are enclosed in purple boxes, with their respective context+ scores. (d) Schematic representation of miR-145 genomic wild-type sequence (top) and CRISPR/Cas9 mutant sequence (bottom). Mature mir-145-5p and -3p in green and orange, respectively, and underlined gRNA sequences are shown. Drosha and Dicer1 cleavage sites marked with red and blue arrows, respectively.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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