FIGURE

FIGURE 7

ID

ZDB-FIG-210718-38

Publication

Hsiao et al., 2021 - The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects

Other Figures

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FIGURE 7

Effects of altering mthfd1L expression on eye development in folate-deficient embryos. (A) FD mthfd1L morphants had their eye size measured at 3 dpf. (B) FD embryos were injected with 0–250 pg zebrafish mthfd1L cRNA and their eye size was measured at 3 dpf. (C)mthfd1L-cRNA-injected control and FD embryos had their OMR estimated at 7 dpf. (D–F) 30 hpf control and FD embryos with or without 250 pg mthfd1L cRNA injection were subjected to HPLC analysis for their content of 10-CHO-THF (D), 5-CH₃-THF (E), and THF/5,10-CH₂-THF (F). Ctl, control; mFD, mild folate deficiency; sFD, severe folate deficiency; *p < 0.05; **p < 0.01; ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mthfd1l WT + MO2-mthfd1l cku1Tg cku1Tg + MO1-mthfd1l cku1Tg + MO2-mthfd1l
Condition:	heat shock
Knockdown Reagents:	MO1-mthfd1l MO2-mthfd1l
Observed In:	eye optomotor response whole organism 5,10-methylenetetrahydrofolic acid whole organism 5-methyltetrahydrofolic acid whole organism 10-formyltetrahydrofolic acid whole organism tetrahydrofolate
Stage Range:	Prim-15 to Days 7-13

Phenotype Detail

Acknowledgments

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