FIGURE

FIGURE 4

ID

ZDB-FIG-210718-35

Publication

Hsiao et al., 2021 - The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects

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FIGURE 4

Larval folate composition and response to rescuing agents. Both control and FD embryos continuously exposed to rescuing agents starting from 25 hpf were examined for their intracellular folate composition (A) at indicated stages with HPLC as described in materials and methods, eye size (B–F) at 3 dpf and OMR (G) at 7 dpf. The rescuing agents include deoxy-ribonucleoside triphosphate (dNTP) (B), Deoxythymidine triphosphate (dTTP) (C), hypoxanthine (Hx) (D), formate (NaF, sodium formate) (E) and N-acetyl-cysteine (NAC) (F). Statistical results are shown in mean ± SEM for (B–F) and percentage in a group for (G). ***p < 0.001; **p < 0.01; *p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cku1Tg
Conditions:	heat shock chemical treatment by environment: 2'-deoxyribonucleoside 5'-triphosphate, heat shock chemical treatment by environment: dTTP, heat shock chemical treatment by environment: sodium formate, heat shock chemical treatment by environment: hypoxanthine, heat shock chemical treatment by environment: N-acetyl-L-cysteine, heat shock
Observed In:	eye optomotor response whole organism 5,10-methylenetetrahydrofolic acid whole organism 5-methyltetrahydrofolic acid whole organism 10-formyltetrahydrofolic acid
Stage Range:	Prim-5 to Days 7-13

Phenotype Detail

Acknowledgments

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