FIGURE 1

Proliferation of radial glia (RG) is increased in response to stab wound injury. Representative images of proliferative RG (BLBP⁺PCNA⁺ cells) in the uninjured (A) and injured (B) hemispheres at 2 days post injury (dpi). (A’,B’) Magnified images of the boxed area in (A,B). (C–F) Representative images of proliferative RG in the injured hemisphere at 6 h post injury (hpi) and at 1, 4, and 7 dpi. White arrowheads indicate BLBP⁺PCNA⁺ cells, and dashed lines indicate injured areas. Scale bar: 50 μm in (A–F) and (A’,B’). Schematic drawing of the stab injury in the right hemisphere of the optic tectum and cross-section. (G) Quantification of proliferative RG in both uninjured and injured hemispheres at 6 (n = 5) and 12 (n = 3) hpi, and 1 (n = 5), 2 (n = 5), 3 (n = 4), 4 (n = 5), and 7 (n = 4) dpi. (H) Quantification of proliferative cells (BLBP^–PCNA⁺ cells) except NE in both uninjured and injured hemispheres at 6 (n = 5) and 12 (n = 3) hpi, and 1 (n = 5), 2 (n = 5), 3 (n = 4), 4 (n = 5), and 7 (n = 4) dpi. Statistical analyses between uninjured and injured hemispheres at each time point were evaluated using paired Student’s t-tests. Statistical significance was defined as *P < 0.05; **P < 0.01; ***P < 0.001.